机构简介

现任领导

胡宝洋

[研究员]

干细胞与神经再生研究组

联系方式 byhu@ioz.ac.cn +86-10-64806251

教育经历

2004年获复旦大学博士学位。

工作经历

2005年赴美国威斯康星大学（University of Wisconsin-Madison）从事博士后研究，2009年任助理科学家（Assistant Scientist），2011年1月加入中国科学院动物研究所。

研究方向

主要从事干细胞与再生医学领域研究，重点研究全能干细胞向神经系的分化机制、人类干细胞生物学的独特性以及干细胞医药学应用的系统化评估等科学问题。

1. 神经分化机制。用多能干细胞定向分化和细胞重编程为模型，研究神经分化中细胞外信号、转录程序、转录前后表观遗传学因素如何协同指导多能干细胞向某一特定类型细胞分化。借助小鼠等动物模型和遗传学手段在体内进一步验证。

2. 人类发育和细胞分化的独特性。以人多能干细胞神经定向分化系统为基础，综合利用多种手段发掘决定人类高级功能和独特性状的调控元件及调控机制。

3. 干细胞医学转化。以定向分化为基础，完善规模化细胞分化和体细胞直接转分化为特定细胞类型的方法。与相关领域合作，建立细胞产品应用于细胞替代和药物筛选的标准评估体系。


代表性发表论文

  1. J. Liu#; Z. Hou#.; J. Wu#; K. Liu; D. Li, T. Gao; W. Liu; B. An; Y. Sun; F. Mo; L. Wang; Y. Wang*; J. Hao*; B. Hu*, Infusion of hESC derived Immunity-and-matrix regulatory cells improves cognitive ability in early-stage AD mice. Cell Prolif 2021, 54 (8), e13085.

  2. J. Wu#; D. Song#; Z. Li#; B. Guo#; Y. Xiao; W. Liu, L. Liang; C. Feng; T. Gao, Y. Chen; Y. Li, Z. Wang, J. Wen, S. Yang, P. Liu, L. Wang, Y. Wang; L. Peng; G. N. Stacey; Z. Hu, G. Feng; W. Li; Y. Huo; R. Jin, N. Shyh-Chang, Q. Zhou, L. Wang; B. Hu*; H. Dai*; J. Hao*, Immunity-and-matrix-regulatory cells derived from human embryonic stem cells safely and effectively treat mouse lung injury and fibrosis. Cell Res 2020,  30 (9), 794-809.

  3. J. Wu; Z. Hu, L. Wang; Y. Tan; W. Hou; Z. Li; T. Gao; J. Fan; B. Guo; H. Dai; W. Li; J. Hao; R. Jin; B. Hu*, First case of COVID-19 infused with hESC derived immunity- and matrix-regulatory cells. Cell Prolif 2020,  53 (12), e12943.

  4. J. Wu#; X. Zhou#; Y. Tan#; L. Wang#; T. Li; Z. Li; T. Gao; J. Fan; B. Guo; W. Li; J. Hao*; X. Wang*; B. Hu*, Phase 1 trial for treatment of COVID-19 patients with pulmonary fibrosis using hESC-IMRCs. Cell Prolif 2020,  53 (12), e12944.

  5. F. Han; B. Hu,  Stem Cell Therapy for Parkinson's Disease. Adv Exp Med Biol 2020,  1266, 21-38

  6. W. Zhu#, B. Zhang#, M. Li#, F. Mo, T. Mi, Y. Wu, Z. Teng, Q. Zhou*, W. Li*, and B. Hu*, Precisely controlling endogenous protein dosage in hPSCs and derivatives to model FOXG1 syndrome. Nat Commun, 2019. 10(1): p. 928. DOI: 10.1038/s41467-019-08841-7

  7. Y. Xu#, Y. Qiu#, B. Zhang#, G. Chen#, Q. Chen, M. Wang, F. Mo, J. Xu, J. Wu, R. Zhang, M. Cheng, N. Zhang, B. Lyu, W. Zhu, M. Wu, Q. Ye, D. Zhang, J. Man, X. Li, J. Cui, Z. Xu, B. Hu*, X. Zhou*, and C. Qin*, Zika virus infection induces RNAi-mediated antiviral immunity in human neural progenitors and brain organoids. Cell Res, 2019. 29(4): p. 265-273. DOI: 10.1038/s41422-019-0152-9

  8. L. Wang, W. Wu, Q. Gu, Z. Liu, Q. Li, Z. Li, J. Fang, W. Liu, J. Wu, Y. Zhang, L. Wang, H. Xu, W. Li, B. Hu, Q. Zhou*, Z. Yin*, and J. Hao*, The effect of clinical-grade retinal pigment epithelium derived from human embryonic stem cells using different transplantation strategies. Protein Cell, 2019. 10(6): p. 455-460. DOI: 10.1007/s13238-018-0606-8

  9. H. Sun, J. Liu, S. Li, L. Zhou, J. Wang, L. Liu*, F. Lv, Q. Gu*, B. Hu, Y. Ma*, and S. Wang*, Reactive Amphiphilic Conjugated Polymers for Inhibiting Amyloid beta Assembly. Angew Chem Int Ed Engl, 2019. 58(18): p. 5988-5993. DOI: 10.1002/anie.201901459

  10. W. Zhu, M. Li, Y. Wu, and B. Hu*, Precise immune tolerance for hPSC derivatives in clinical application. Cell Immunol, 2018. 326: p. 15-23. DOI: 10.1016/j.cellimm.2017.08.005

  11. W. Zhang#, H. Wan#, G. Feng#, J. Qu#, J. Wang, Y. Jing, R. Ren, Z. Liu, L. Zhang, Z. Chen, S. Wang, Y. Zhao, Z. Wang, Y. Yuan, Q. Zhou, W. Li*, G.H. Liu*, and B. Hu*, SIRT6 deficiency results in developmental retardation in cynomolgus monkeys. Nature, 2018. 560(7720): p. 661-665. DOI: 10.1038/s41586-018-0437-z

  12. B. Zhang#, Y. He#, Y. Xu#, F. Mo, T. Mi, Q. Shen, C. Li, Y. Li, J. Liu, Y. Wu, G. Chen, W. Zhu, C. Qin*, B. Hu*, and G. Zhou*, Differential antiviral immunity to Japanese encephalitis virus in developing cortical organoids. Cell Death Dis, 2018. 9(7): p. 719. DOI: 10.1038/s41419-018-0763-y

  13. M. Wu, D. Zhang, C. Bi, T. Mi, W. Zhu, L. Xia, Z. Teng, B. Hu*, and Y. Wu*, A Chemical Recipe for Generation of Clinical-Grade Striatal Neurons from hESCs. Stem Cell Reports, 2018. 11(3): p. 635-650. DOI: 10.1016/j.stemcr.2018.08.005

  14. Y. Wang#, W. Zhu#, M. Wu#, Y. Wu, Z. Liu, L. Liang, C. Sheng, J. Hao, L. Wang, W. Li, Q. Zhou*, B. Hu*, Human Clinical-Grade Parthenogenetic ESC-Derived Dopaminergic Neurons Recover Locomotive Defects of Nonhuman Primate Models of Parkinson's Disease. Stem Cell Reports, 2018. 11(1): p. 171-182. DOI: 10.1016/j.stemcr.2018.05.010

  15. X. #Wang, T. Li#, T. Cui#, D. Yu#, C. Liu#, L. Jiang, G. Feng, L. Wang, R. Fu, X. Zhang, J. Hao, Y. Wang, L. Wang, Q. Zhou*, W. Li*, and B. Hu*, Human embryonic stem cells contribute to embryonic and extraembryonic lineages in mouse embryos upon inhibition of apoptosis. Cell Res, 2018. 28(1): p. 126-129. DOI: 10.1038/cr.2017.138

  16. Y. Tan#, P. Han#, Q. Gu#, G. Chen, L. Wang, R. Ma, J. Wu, C. Feng, Y. Zhang, L. Wang, B. Hu, W. Li, J. Hao*, and Q. Zhou*, Generation of clinical-grade functional cardiomyocytes from human embryonic stem cells in chemically defined conditions. J Tissue Eng Regen Med, 2018. 12(1): p. 153-163. DOI: 10.1002/term.2381

  17. Z. Li#, L. Wang#, L. Wang#, G. Feng#, X. Yuan#, C. Liu, K. Xu, Y. Li, H. Wan, Y. Zhang, Y. Li, X. Li, W. Li*, Q. Zhou*, B. Hu*, Generation of Bimaternal and Bipaternal Mice from Hypomethylated Haploid ESCs with Imprinting Region Deletions. Cell Stem Cell, 2018. 23(5): p. 665-676 e4. DOI: 10.1016/j.stem.2018.09.004

  18. X. Li#, R. Ma#, Q. Gu#, L. Liang, L. Wang, Y. Zhang, X. Wang, X. Liu, Z. Li, J. Fang, J. Wu, Y. Wang, W. Li, B. Hu, L. Wang*, Q. Zhou*, and J. Hao*, A fully defined static suspension culture system for large-scale human embryonic stem cell production. Cell Death Dis, 2018. 9(9): p. 892. DOI: 10.1038/s41419-018-0863-8

  19. Y. Jiang#, L. Yan#, L. Xia, X. Lu, W. Zhu, D. Ding, M. Du, D. Zhang, H. Wang*, and B. Hu*, Zinc finger E-box-binding homeobox 1 (ZEB1) is required for neural differentiation of human embryonic stem cells. J Biol Chem, 2018. 293(50): p. 19317-19329. DOI: 10.1074/jbc.RA118.005498

  20. G. Chen#, D. Zhang#, L. Zhang#, G. Feng, B. Zhang, Y. Wu, W. Li, Y. Zhang*, and B. Hu*, RBM14 is indispensable for pluripotency maintenance and mesoderm development of mouse embryonic stem cells. Biochem Biophys Res Commun, 2018. 501(1): p. 259-265. DOI: 10.1016/j.bbrc.2018.04.231

  21. L. Yuan#, X. Huang#, Z. Liu#, F. Zhang#, X. Zhu#, J. Yu#, X. Ji, Y.P. Xu, G. Li, C. Li, H. Wang, Y. Deng, M. Wu, M. Cheng, Q. Ye, D. Xie, X. Li, X. Wang, W. Shi, B. Hu, P.Y. Shi, Z. Xu*, and C. Qin*, A single mutation in the prM protein of Zika virus contributes to fetal microcephaly. Science, 2017. 358(6365): p. 933-936. DOI: 10.1126/science.aam7120

  22. L. Yan#, Y. Li#, Z. Shi#, X. Lu#, J. Ma, B. Hu, J. Jiao*, and H. Wang*, The zinc finger E-box-binding homeobox 1 (Zeb1) promotes the conversion of mouse fibroblasts into functional neurons. J Biol Chem, 2017. 292(31): p. 12959-12970. DOI: 10.1074/jbc.M116.771493

  23. K. Qian#, H. Huang#, A. Peterson, B. Hu, N.J. Maragakis, G.L. Ming, H. Chen*, and S.C. Zhang*, Sporadic ALS Astrocytes Induce Neuronal Degeneration In Vivo. Stem Cell Reports, 2017. 8(4): p. 843-855. DOI: 10.1016/j.stemcr.2017.03.003

  24. P. Liu#, K. Liu#, H. Gu, W. Wang, J. Gong, Y. Zhu, Q. Zhao, J. Cao, C. Han, F. Gao, Q. Chen, W. Li, J. Jiao, B. Hu, Q. Zhou, and T. Zhao*, High autophagic flux guards ESC identity through coordinating autophagy machinery gene program by FOXO1. Cell Death Differ, 2017. 24(10): p. 1672-1680. DOI: 10.1038/cdd.2017.90

  25. T. Li#, G. Feng#, Y. Li#, M. Wang#, J. Mao, J. Wang, X. Li, X. Wang, B. Qu, L.Y. Wang, X. Zhang, H. Wan, T. Cui, C. Wan, L. Liu, X. Zhao, B. Hu, W. Li, and Q. Zhou*, Rat embryonic stem cells produce fertile offspring through tetraploid complementation. Proc Natl Acad Sci U S A, 2017. 114(45): p. 11974-11979. DOI: 10.1073/pnas.1708710114

  26. Q. Gu#, J. Wang#, L. Wang#, Z. Liu, W. Zhu, Y. Tan, W. Han, J. Wu, C. Feng, J. Fang, L. Liu, L. Wang, W. Li, X. Zhao, B. Hu, J. Hao*, and Q. Zhou*, Accreditation of Biosafe Clinical-Grade Human Embryonic Stem Cells According to Chinese Regulations. Stem Cell Reports, 2017. 9(1): p. 366-380. DOI: 10.1016/j.stemcr.2017.04.017

  27. J. Zhang#, S. Chen#, D. Zhang, Z. Shi, H. Li, T. Zhao, B. Hu, Q. Zhou, and J. Jiao*, Tet3-Mediated DNA Demethylation Contributes to the Direct Conversion of Fibroblast to Functional Neuron. Cell Rep, 2016. 17(9): p. 2326-2339. DOI: 10.1016/j.celrep.2016.10.081

  28. C. Zhang, J. Cao, X. Li, H. Xu, W. Wang, L. Wang, X. Zhao, W. Li, J. Jiao, B. Hu, Q. Zhou*, and T. Zhao*, Treatment of multiple sclerosis by transplantation of neural stem cells derived from induced pluripotent stem cells. Sci China Life Sci, 2016. 59(9): p. 950-7. DOI: 10.1007/s11427-016-0114-9

  29. Z. Shi, J. Zhang, S. Chen, Y. Li, X. Lei, H. Qiao, Q. Zhu, B. Hu, Q. Zhou, and J. Jiao*, Conversion of Fibroblasts to Parvalbumin Neurons by One Transcription Factor, Ascl1, and the Chemical Compound Forskolin. J Biol Chem, 2016. 291(26): p. 13560-70. DOI: 10.1074/jbc.M115.709808

  30. K. Liu#, Q. Zhao#, P. Liu#, J. Cao, J. Gong, C. Wang, W. Wang, X. Li, H. Sun, C. Zhang, Y. Li, M. Jiang, S. Zhu, Q. Sun, J. Jiao, B. Hu, X. Zhao, W. Li, Q. Chen, Q. Zhou*, and T. Zhao*, ATG3-dependent autophagy mediates mitochondrial homeostasis in pluripotency acquirement and maintenance. Autophagy, 2016. 12(11): p. 2000-2008. DOI: 10.1080/15548627.2016.1212786

  31. Z. Li#, H. Wan#, G. Feng#, L. Wang#, Z. He, Y. Wang, X.J. Wang, W. Li*, Q. Zhou*, and B. Hu*, Birth of fertile bimaternal offspring following intracytoplasmic injection of parthenogenetic haploid embryonic stem cells. Cell Res, 2016. 26(1): p. 135-8. DOI: 10.1038/cr.2015.151

  32. Y. Jiang#, M. Du#, M. Wu, Y. Zhu, X. Zhao, X. Cao, X. Li, P. Long, W. Li*, and B. Hu*, Phosphatidic Acid Improves Reprogramming to Pluripotency by Reducing Apoptosis. Stem Cells Dev, 2016. 25(1): p. 43-54. DOI: 10.1089/scd.2015.0159

  33. L. Gao, Y. Zhang, B. Hu, J. Liu, P. Kong, S. Lou, Y. Su, T. Yang, H. Li, Y. Liu, C. Zhang, L. Gao, L. Zhu, Q. Wen, P. Wang, X. Chen, J. Zhong, and X. Zhang*, Phase II Multicenter, Randomized, Double-Blind Controlled Study of Efficacy and Safety of Umbilical Cord-Derived Mesenchymal Stromal Cells in the Prophylaxis of Chronic Graft-Versus-Host Disease After HLA-Haploidentical Stem-Cell Transplantation. J Clin Oncol, 2016. 34(24): p. 2843-50. DOI: 10.1200/JCO.2015.65.3642

  34. H. Zhu#, Y. Tan#, Q. Gu, W. Han, Z. Li, J. S. Meyer*, and B. Hu*, Regulations in the United States for cell transplantation clinical trials in neurological diseases. Translational Neuroscience and Clinics, 2015. 1(2): p. 114-124. DOI: 10.18679/cn11-6030/r.2015.015

  35. L. Wang#, J. Cao#, Y. Wang#, T. Lan#, L. Liu, W. Wang, N. Jin, J. Gong, C. Zhang, F. Teng, G. Yan, C. Li, J. Li, H. Wan, B. Hu, W. Li, X. Zhao, Z. Qi*, T. Zhao*, and Q. Zhou*, Immunogenicity and functional evaluation of iPSC-derived organs for transplantation. Cell Discov, 2015. 1: p. 15015. DOI: 10.1038/celldisc.2015.15

  36. H. Wan#, C. Feng#, F. Teng#, S. Yang#, B. Hu, Y. Niu, A.P. Xiang, W. Fang, W. Ji, W. Li*, X. Zhao*, and Q. Zhou*, One-step generation of p53 gene biallelic mutant Cynomolgus monkey via the CRISPR/Cas system. Cell Res, 2015. 25(2): p. 258-61. DOI: 10.1038/cr.2014.158

  37. W. Shi#, L.I. Zhu#, Y. Wang, B. Hu, H. Xiao, G. Zhou, and C. Chen*, Estrogen therapy to treat retinopathy in newborn mice. Exp Ther Med, 2015. 10(2): p. 611-617. DOI: 10.3892/etm.2015.2554

  38. H. Chen*, K. Qian, W. Chen, B. Hu, L.W.t. Blackbourn, Z. Du, L. Ma, H. Liu, K.M. Knobel, M. Ayala, and S.C. Zhang*, Human-derived neural progenitors functionally replace astrocytes in adult mice. J Clin Invest, 2015. 125(3): p. 1033-42. DOI: 10.1172/JCI69097

  39. S. Wang, W. Li, S. Wang, and B. Hu*, Rapid and efficient assembly of transcription activator-like effector genes by USER cloning. J Genet Genomics, 2014. 41(6): p. 339-47. DOI: 10.1016/j.jgg.2014.05.002

  40. L. Cong, H. Xiao, B. Hu*, and G. Zhou*, Drastic alleviation of neovascularization in oxygen induced retinopathy of mice following intravitreal bone marrow stromal cell transplantation. Chinese Journal of Neuroanatomy, 2014. 30(3): p. 249-260.

  41. M. Wu, G. Chen, and B. Hu*, Induced pluripotency for translational research. Genomics Proteomics Bioinformatics, 2013. 11(5): p. 288-93. DOI: 10.1016/j.gpb.2013.08.001

  42. L. Ma, B. Hu, Y. Liu, S.C. Vermilyea, H. Liu, L. Gao, Y. Sun, X. Zhang, and S.C. Zhang*, Human embryonic stem cell-derived GABA neurons correct locomotion deficits in quinolinic acid-lesioned mice. Cell Stem Cell, 2012. 10(4): p. 455-64. DOI: 10.1016/j.stem.2012.01.021

  43. Y. Jiang, M. Zhang, B. Hu*, Specification of functional neurons and glia from human pluripotent stem cells. Protein Cell, 2012. 3(11): p. 818-25. DOI: 10.1007/s13238-012-2086-6

  44. J. Hao, D. Campagnolo, R. Liu, W. Piao, S. Shi, B. Hu, R. Xiang, Q. Zhou, T. Vollmer, L. Van Kaer, A. La Cava, and F.D. Shi*, Interleukin-2/interleukin-2 antibody therapy induces target organ natural killer cells that inhibit central nervous system inflammation. Ann Neurol, 2011. 69(4): p. 721-34. DOI: 10.1002/ana.22339

  45. B. Hu and S. Zhang, Directed Differentiation of Neural-stem cells and Subtype-Specific Neurons from hESCs, in Cellular Programming and Reprogramming: Methods and Protocols, S. Ding, Editor. 2010, Humana Press: Totowa, NJ. p. 123-137.

  46. B. Hu, J. Weick, J. Yu, L. Ma, X. Zhang, J. Thomson, and S. Zhang*, Neural differentiation of human induced pluripotent stem cells follows developmental principles but with variable potency. Proc Natl Acad Sci U S A, 2010. 107(9): p. 4335-40. DOI: 10.1073/pnas.0910012107

  47. B. Hu and S. Zhang*, Differentiation of spinal motor neurons from pluripotent human stem cells. Nat Protoc, 2009. 4(9): p. 1295-304. DOI: 10.1038/nprot.2009.127

  48. B. Hu, Z. Du, and S. Zhang*, Differentiation of human oligodendrocytes from pluripotent stem cells. Nat Protoc, 2009. 4(11): p. 1614-22. DOI: 10.1038/nprot.2009.186

  49. B. Hu#, Z. Du#, X. Li, M. Ayala, and S. Zhang*, Human oligodendrocytes from embryonic stem cells: conserved SHH signaling networks and divergent FGF effects. Development, 2009. 136(9): p. 1443-52. DOI: 10.1242/dev.029447

  50. Z. Du, B. Hu, M. Ayala, B. Sauer, and S. Zhang*, Cre recombination-mediated cassette exchange for building versatile transgenic human embryonic stem cells lines. Stem Cells, 2009. 27(5): p. 1032-41. DOI: 10.1002/stem.38

  51. X. Li, B. Hu, S. Jones, Y. Zhang, T. Lavaute, Z. Du, and S. Zhang*, Directed differentiation of ventral spinal progenitors and motor neurons from human embryonic stem cells by small molecules. Stem Cells, 2008. 26(4): p. 886-93. DOI: 10.1634/stemcells.2007-0620

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