论文题目：Development and Application of CRISPR-Cas Based Tools

作者: Hu Y, Li W

日期：2022.4

发表期刊：Front Cell Dev Biol

doi：10.3389/fcell.2022.834646

摘要：

  Abundant CRISPR-Cas systems in nature provide us with unlimited valuable resources to develop a variety of versatile tools, which are powerful weapons in biological discovery and disease treatment. Here, we systematically review the development of CRISPR-Cas based tools from DNA nuclease to RNA nuclease, from nuclease dependent-tools to nucleic acid recognition dependent-tools. Also, considering the limitations and challenges of current CRISPR-Cas based tools, we discuss the potential directions for development of novel CRISPR toolkits in the future.