科研成果

Development and Application of CRISPR-Cas Based Tools

2022-04-04

论文题目:Development and Application of CRISPR-Cas Based Tools

作者: Hu Y, Li W

日期:2022.4

发表期刊:Front Cell Dev Biol

doi:10.3389/fcell.2022.834646

摘要:

  Abundant CRISPR-Cas systems in nature provide us with unlimited valuable resources to develop a variety of versatile tools, which are powerful weapons in biological discovery and disease treatment. Here, we systematically review the development of CRISPR-Cas based tools from DNA nuclease to RNA nuclease, from nuclease dependent-tools to nucleic acid recognition dependent-tools. Also, considering the limitations and challenges of current CRISPR-Cas based tools, we discuss the potential directions for development of novel CRISPR toolkits in the future.



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