研究员-- 干细胞与再生医学创新研究院

研究队伍

研究员

郭帆

[研究员]

生殖表观遗传研究组

联系方式 guofan@ioz.ac.cn

教育经历

2008年获得武汉大学学士学位，2014年获得中国科学院大学博士学位。

工作经历

2014年起在北京大学从事博士后研究工作；2017年3月至2021年4月在四川大学担任研究员、课题组长、博士生导师；2021年5月起任中科院动物所研究员、生殖表观遗传研究组组长。

研究方向

主要研究哺乳动物早期胚胎发育与生殖细胞发生过程中的表观遗传调控。

表观遗传调控在哺乳动物发育过程中至关重要，其对基因转录活性、染色质状态以及高级结构的改变将影响多能性的建立与维持、细胞命运决定与谱系分化、细胞稳态维持与功能等。虽然多种类型的表观遗传修饰（如DNA修饰、组蛋白修饰）及其相关调节因子（如修饰酶、去修饰酶）已被报道，但其在生理状态下的动态与功能仍有待深入研究。我们实验室将利用前沿的分子生物学方法、成像工具和在体模型等来研究：1. 生殖细胞发生与胚胎发育中的表观遗传编程和重编程；2. 不同表观遗传修饰之间的互作对细胞稳态与功能的影响；3. 细胞命运决定与转变过程中的表观遗传调控。

代表性发表论文

Yan, R., C. Gu*, D. You, Z. Huang, J. Qian, Q. Yang, X. Cheng, L. Zhang, H. Wang, P. Wang* and F. Guo* (2021). "Decoding dynamic epigenetic landscapes in human oocytes using single-cell multi-omics sequencing." Cell Stem Cell 28(9): 1641-1656.
Long, X., Q. Yang, J. Qian, H. Yao, R. Yan, X. Cheng, Q. Zhang, C. Gu, F. Gao, H. Wang, L. Zhang* and F. Guo* (2021). "Obesity modulates cell-cell interactions during ovarian folliculogenesis." iScience 25(1): 103627.
Yang, Z., X. Xu, C. Gu, J. Li, Q. Wu, C. Ye, A. V. Nielsen, L. Mao, J. Ye, K. Bai, F. Guo*, C. Tang* and Y. Zhao* (2020). "Chemicals orchestrate reprogramming with hierarchical activation of master transcription factors primed by endogenous Sox17 activation." Communications Biology 3(1): 629.
Zhou, C., Y. Sun, R. Yan, Y. Liu, E. Zuo, C. Gu, L. Han, Y. Wei, X. Hu, R. Zeng, Y. Li*, H. Zhou*, F. Guo* and H. Yang* (2019). "Off-target RNA mutation induced by DNA base editing and its elimination by mutagenesis." Nature 571(7764): 275-278.
Gu, C., S. Liu, Q. Wu, L. Zhang and F. Guo* (2019). "Integrative single-cell analysis of transcriptome, DNA methylome and chromatin accessibility in mouse oocytes." Cell Research 29(2): 110-123. Cover story
Chen, X., J. Zhao, C. Gu, Y. Cui, Y. Dai, G. Song, H. Liu, H. Shen, Y. Liu, Y. Wang, H. Xing, X. Zhu, P. Hao, F. Guo* and X. Liu* (2018). "Med23 serves as a gatekeeper of the myeloid potential of hematopoietic stem cells." Nature Communications 9(1): 3746.
Li, L., F. Guo, Y. Gao, Y. Ren, P. Yuan, L. Yan, R. Li, Y. Lian, J. Li, B. Hu, J. Gao, L. Wen, F. Tang and J. Qiao (2018). "Single-cell multi-omics sequencing of human early embryos." Nature Cell Biology 20(7): 847-858.
Guo, F.*, L. Li, J. Li, X. Wu, B. Hu, P. Zhu, L. Wen and F. Tang* (2017). "Single-cell multi-omics sequencing of mouse early embryos and embryonic stem cells." Cell Research 27(8): 967-988. Cover story
Guo, F., L. Yan, H. Guo, L. Li, B. Hu, Y. Zhao, J. Yong, Y. Hu, X. Wang, Y. Wei, W. Wang, R. Li, J. Yan, X. Zhi, Y. Zhang, H. Jin, W. Zhang, Y. Hou, P. Zhu, J. Li, L. Zhang, S. Liu, Y. Ren, X. Zhu, L. Wen, Y. Q. Gao, F. Tang and J. Qiao (2015). "The Transcriptome and DNA Methylome Landscapes of Human Primordial Germ Cells." Cell 161(6): 1437-1452. Cover story
Guo, F., X. Li, D. Liang, T. Li, P. Zhu, H. Guo, X. Wu, L. Wen, T. P. Gu, B. Hu, C. P. Walsh, J. Li, F. Tang and G. L. Xu (2014). "Active and passive demethylation of male and female pronuclear DNA in the mammalian zygote." Cell Stem Cell 15(4): 447-459.
Gu, T. P., F. Guo, H. Yang, H. P. Wu, G. F. Xu, W. Liu, Z. G. Xie, L. Shi, X. He, S. G. Jin, K. Iqbal, Y. G. Shi, Z. Deng, P. E. Szabo, G. P. Pfeifer, J. Li and G. L. Xu (2011). "The role of Tet3 DNA dioxygenase in epigenetic reprogramming by oocytes." Nature 477(7366): 606-610.

*Corresponding author